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| 名称 | KYSE-410 (人食管鳞癌细胞) (STR鉴定正确) |
| 别称 | KYSE 410; KYSE410; KYSE0410 |
| 背景描述 | Established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from the cervical esophagus of a 51-year-old Japanese man prior to treatment (tumor invasion into the adventitia was obvious); described in the literature to overexpress hst-1 (= heparin-binding growth factor) and cyclin D1 |
| 种属 | 人 |
| 年龄（性别） | 女；51岁 |
| 组织来源 | 食管鳞癌 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 食管癌细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 推荐传代比例 | 1:4-1:6 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~32-45 hours |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 保藏机构 | DSMZ; ACC-381 ECACC; 94072023 JCRB; JCRB1419 |
STR位点信息
| Amelogenin | X |
| CSF1PO | 12 |
| D2S1338 | 17,19 |
| D3S1358 | 15,16 |
| D5S818 | 13 |
| D7S820 | 12 |
| D8S1179 | 10 |
| D13S317 | 11 |
| D16S539 | 10,12 |
| D18S51 | 13,15 |
| D19S433 | 13 |
| D21S11 | 30 |
| FGA | 20 |
| PentaD | 11 |
| PentaE | 8,12 |
| TH01 | 8 |
| TPOX | 8,11 |
| vWA | 16,18 |
| D6S1043 | 13,15 |
| D12S391 | 17,19 |
| D2S441 | 11,12 |
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